Reference library
Replicative DNA Polymerase δ but Not ε Proofreads Errors in Cis and in Trans
Paper summary
Yeast defined-base-pair reversion assays combine proofreading-defective polymerases with mismatch-repair deficiency. Overlapping proofreading and mismatch-repair layers suppress errors, and polymerase δ can proofread errors in trans whereas ε chiefly acts in cis in these assays. Certain 8-oxoG-associated mismatches escape proofreading. Reporter orientation and local sequence context constrain the inference; this is not a genome-wide universal mutation-rate estimate.
Relevance to the project
Assistant interpretation: biological fidelity layers motivate separate digital checks for valid inheritance, controlled variation and post-variation behavior. DNA error correction does not evaluate ecological usefulness and should not be equated with an optimization objective.
Targeted yeast reporter experiments; not a complete audit or extrapolation to all organisms. Chemical damage can evade safeguards. Human disease implications are outside this project research.
Source record
- Reference ID
- polymerase-proofreading-cis-trans-2015
- Archived version
- Publisher printable PDF, published 5 March 2015; 32 pages
- Summary provenance
- reference-library/papers/polymerase-proofreading-cis-trans-2015/metadata.json · summary
- Review status
- Targeted primary full-text review; not exhaustive reading or reproduction
- Reading scope
- Publisher HTML abstract, author summary, introduction, TRP5 reversion results and Fig.2 caption, discussion opening/limits, 8-oxoG negative result and reversion analysis; PDF opening identity and pp.2,19,25–26 checked. Not all 32 pages, figures or supplementary evidence read.